By Gorm Palmgren – CRISPR News Medicine –
NGPS is a rare premature ageing disorder caused by a mutation in BANF1, encoding barrier-to-autointegration factor (BAF), a nuclear envelope-associated protein. Using patient-derived fibroblasts, researchers conducted a CRISPR-Cas9-based synthetic rescue screen to identify genes whose loss could reverse key NGPS cellular phenotypes.
The CRISPR screen was designed to assess the effect of gene knockouts on four NGPS-specific phenotypes: nuclear shape abnormalities, mislocalisation of emerin, increased nuclear envelope ruptures, and micronuclei formation (see Figure 1). The researchers employed a microscopy-based approach to quantify these features in fibroblasts stably expressing Cas9.
