By Science Direct –
Abstract
Mesenchymal stem cells (MSCs) possess remarkable capabilities including multipotency, proliferation, and regeneration. Reaching to the higher numbers of passages in the cell culture is essential for providing enough reservoir for the clinical application. However, these cells undergo an aging process during long cultures. Decline of proliferation, stemness, and differentiation potentials, impaired homing, and decreased immunomodulatory capacity are main biological changes in MSCs aging. Thus, aging of MSCs during long cultures is a bottle neck for regenerative medicine and cell-therapy. The preconditioning of MSCs with herbal extracts has been used for multiple purposes, such as decreasing the adverse effects of chemotherapeutics, increasing migration of MSCs, rescuing MSCs against oxidative stress, and enhancing the therapeutic efficiency of them. Besides researchers have been used herbal preconditioning as an innovative and feasible approach to delay or reverse the senescence of MSCs, during in vitro propagation. This review highlights the morphological, biological, phenotypic, and stemness changes observed in senescent MSCs. Subsequently, the mechanisms underlying MSCs senescence have been scrutinized. The novelty of the current work is that strategies and mechanisms to inhibit MSCs aging or rejuvenate them by herbal pretreatment will be dissected.
